Faculty Profile

Dr. Sandeep Singh
Associate Professor
Department of Human Genetics and Molecular Medicine
School of Health Sciences
Central University of Punjab
Bathinda- 151001
Email Id: sandeepsingh82@cup.edu.in; sandeep@cup.ac.in
Mobile: +91 9463563806
 
Education :
Degree Name of the Institute Year Subjects
PhD National Centre for Cell Science, Pune, India 2010 Biotechnology
M.Sc. National Centre for Human Genome Studies and Research, Panjab University, Chandigarh 2004 Human Genomics
B.Sc. Punjabi University, Patiala 2002 Biology
Research and Teaching Experience :
Name of the Institute Position Period
Department of Human Genetics and Molecular Medicine, School of Health Sciences, Central University of Punjab, Bathinda Assistant Professor 2013 - 2020
Centre for Human Genetics,School of Health Sciences, Central University of Punjab, Bathinda Assistant Professor 2012 - 2013
Centre for Biosciences, Central University of Punjab, Bathinda Research Associate 2011 - 2012
Hematopoietic stem cells and leukemia research, atCity of Hope, Duarte, California, USA, with aim to delineate the molecular mechanisms associated with inversion-16 recombinantprotein in Acute Myeloid Leukemia stem cells Postdoctoral fellow 2010 - 2011
The field of Chromatin-Cancer biology at National Center for Cell Science(NCCS), Pune, India, under the guidance of Dr. Samit Chattopadhyay

To study SMAR1 interacting partners by yeast two hybrid and its role in cellular differentiation and tumorigenesis.

Ph.D. 2005 - 2010
PGIMER, Chandigarh in NIH funded project Marijuana Abuse: cognition, Neuroimaging and Endocrinecorrelating SNPs and serum markerswith drug abuse with Prof. Madhu Khullar Research Staff 2004 - 2005
Differential regulation of apoptosis in male and female murine peritoneal macrophages With Prof.  Tapas Mukhopadhyay M.Sc. Thesis 2002 - 2004
Area Specializations/Research Interest:
  • Mitochondria and Cancer
  • Mechnisms of carcinogenesis: role of environmental and genetic factors in carcinogenesis
  • Exploration of  natural and synthetic compounds for their anticancer potential
Publications :
  1. Yadav UP, Singh T, Kumar P, Sharma P, Kaur H, Sharma S, Singh S, Kumar S, Mehta K. Metabolic Adaptations in Cancer Stem Cells. Frontiers Oncology, 10:1010. doi: 10.3389/fonc.2020.01010.
  2. Sharma P and Singh S. Combinatorial effect of DCA and let 7a on triple negative MDA-MB-231 Cells: a metabolic approach of treatment. Integrative Cancer Therapies, 19: 1–11. 2020.
  3. Ansari AJ, Joshi G, Yadav U, Maurya AK, Agnihotri V, Kalra S, Kumar R, Singh S, Sawant D. Exploration of Pd-catalysed four-component tandem reaction for one-pot assembly of pyrazolo[1,5-c]quinazolines as potential EGFR inhibitors. Bioorganic Chemistry. 2019 Sep 26;93:103314.
  4. Joshi G, Kalra S, Yadav UP, Sharma P, Singh PK, Amrutkar S, Ansari AJ, Kumar S, Sharon A, Sharma S, Sawant DM, Banerjee UC, Singh S and Kumar R. E-pharmacophore guided discovery of pyrazolo[1,5-c]quinazolines as dual inhibitors of topoisomerase-I and histone deacetylase. Bioorganic Chemistry. 2020 Jan;94:103409.
  5. Ahluwalia TS, Kilpelainen TO, Singh S and Rossingh P. Novel Biomarkers for Type 2 Diabetes. Front. Endocrinol. doi: 10.3389/fendo.2019.00649. Editorial
  6. Joshi G, Kaur J, Sharma P, Kaur G, Bhandari Y, Kumar R and Singh S. p53-Mediated Anticancer Activity of Citrullus colocynthis Extracts. The Natural Product Journal. Accepted. DOI:10.2174/2210315509666181203114329
  7. Sharma P, Kumar B, Dogra N, Singh S. Small regulatory molecules acting big in cancer: Potential Role of mito-miRs in cancer. Current Molecular Medicine, 2019. doi: 10.2174/1566524019666190723165357.
  8. R Mehra, S Bhushan, F Bast and S Singh. Marine macroalga Caulerpa: role of its metabolites in modulating cancer signaling. Molecular Biology Reports. 2019 Jun;46(3):3545-3555
  9. Ansari AJ, Joshi G, Sharma P, Maurya AK, Metre RK, Agnihotri VK, Chandaluri CG, Kumar R, Singh S and Sawant D. Pd-Catalyzed Four-Component Sequential Reaction Delivers a 
Modular Fluorophore Platform for Cell Imaging. J Org Chem. 2019 Apr 5;84(7):3817-3825
  10. Annu, Ahmed S, Kaur G, Sharma P, Singh S, Ikram S. Evaluation of antioxidant, antibacterial and anticancer (lung cancer cell line A549) activity of Punicagranatum mediated silver nanoparticles. Toxicol Res (Camb). 2018 29;7(5):923-930
  11. R Mehra, S Bhushan, UP Yadav, F Bast and S Singh. Caulerpa taxifolia inhibits cell proliferation and induces oxidative stress in breast cancer cells. Biologia 74 (2), 187-193.
  12. Shridhar K, Singh G, Dey S, Dhatt SS, Gill JPS, Goodman M, Magsumbol MS, Pearce N, Singh S, Singh A, Singh P, Thakur JS and Dhillon PK. Dietary Patterns and Breast Cancer Risk:
A Multi-Centre Case Control Study among North Indian Women. Int J Environ Res Public Health. 2018 Sep 6;15(9)
  13. G, Dogra N andSingh S. Health Risk Assessment of Occupationally Pesticide-Exposed Population of Cancer Prone Area of Punjab. Toxicological Sciences. Toxicol Sci. 2018 Sep 1;165(1):157-169.
  14. Kaur G, Sharma P, Dogra N and Singh S. Eradicating Cancer Stem Cells: Concept, Issues, and Challenges. Curr Treat Options Oncol. 2018 Mar 20;19(4):20
  15. De I, Dogra N and Singh S. The mitochondrial unfolded protein response : Role in cellular homeostasis and disease. Current Molecular Medicine 2017, 17(9): 587-597(11)
  16. Sharma, S., Joshi, G., Kalra, S., Singh, S. and Kumar, R., (2018). Synthetic versus Enzymatic Pictet-Spengler Reaction: An Overview. Current Organic Synthesis. 15(7), pp.924-939
  17. Kumar B, Sharma P, Gupta VK, Khullar M, Singh S, Dogra N and Kumar V. Synthesis and Biological Evaluation of Pyrimidine Bridged Biphenyls as Potential Anticancer Agents and Mechanistic Studies. Bioorg Chem. 2018 Aug;78:130-140.
  18. Annu, Ahmad S, Kaur G, Sharma P, Singh S, Ikram S. Fruit Waste (Peel) as Bio-Reductant to synthesize Silver Nanoparticles with Antimicrobial, Antioxidant and Cytotoxic Activities. J Appl Biomed. 2018;16(3):221-231
  19. Kumar B, Singh S, Skvortsova II, Kumar V. Promising Targets in Anti-cancer Drug Development: Recent Updates.Curr Med Chem. 2017 Mar 31. doi: 10.2174/0929867324666170331123648. Kaur G, Jain AK, Singh S. CYP/PON genetic variations as determinant of organophosphate pesticides toxicity. J. Genetics. 2017.
  20. Ahmad M., Manzoor K., Singh S., and Ikram, S. Chitosan centeredbionanocomposites for medical specialty and curative applications: A review. International Journal of Pharmaceutics, (2017). 529(1-2), 200-217
  21. Kaur G, Singh, S and Jain, AK. Pesticide induced cytotoxicity analysis in human cells.Jounal of Agroecology and Natural Resource Management, (2017)4: 44-48.
  22. Joshi, G, Nayyar, H, Kalra, S, Sharma, P, Munshi, A, Singh, S, and Kumar, R. Pyrimidine Containing Epidermal Growth Factor Receptor Kinase inhibitors: Synthesis and Biological Evaluation. Chemical Biology & Drug Design, (2017).
  23. Chauhan, M, Joshi, G, Kler, H, Kashyap, A, Amrutkar, S. M, Sharma, P, Banerjee UC, Bhilare, KD, Singh S and Kumar, R. Dual inhibitors of epidermal growth factor receptor and topoisomerase IIα derived from a quinoline scaffold. RSC Advances, (2016). 6(81), 77717-77734.
  24. Darpan, Joshi, G., Amrutkar, S. M., Baviskar, A. T., Kler, H., Singh, S, Banerjee UC, Kumar, R. Synthesis and biological evaluation of new 2,5-dimethylthiophene/furan based N-acetyl pyrazolines as selective topoisomerase II inhibitors. RSC Advances, (2016). 6(18), 14880-14892. doi:10.1039/c5ra25705k
  25. Raut SK, Singh GB, Rastogi B, Saikia UN, Mittal A, Dogra N, Singh S, Prasad R and Khullar M. miR-30c and miR-181a synergistically modulate p53-p21 pathway in diabetes induced cardiac hypertrophy. Mol Cell Biochem, 2016. PMID: 27221738.
  26. Qi Jing, Singh S, Wei- Kai H, Qi C, Li L, YinWei H, Mcdonald T, Lin A, Guido M, Bhatia R, Chang C, and Kuo YH. HDAC8 Inhibition Specifically Targets Inv(16) Acute Myeloid Leukemic Stem Cells by Restoring p53 Acetylation. Cell Stem Cells. 2015. 17(5): 597-610.
  27. Joshi G, Singh S, Kumar R. Growth factors mediated cell signalling in prostate cancer progression: Implications in discovery of anti-prostate cancer agents. Chem-Biol Interact. (2015). 240:120-33.
  28. Kumar R, Santos CD, Ahluwalia T and Singh S. Signal Transduction Inhibitors as Promising Anticancer Agents," BioMed Research International. (2015). doi:10.1155/2015/584170. Editorial
  29. Negi A, Alex JM, Amrutkar SM, Baviskar AT, Joshi G, Singh S, and Kumar R. Imine/amide–imidazole conjugates derived from 5-amino-4-cyano-N1-substituted benzyl imidazole: Microwave-assisted synthesis and anticancer activity via selective topoisomerase-II-α inhibition. Bioorganic & medicinal chemistry. 2015. 23(17), 5654-5661.
  30. Vikram A, Nand, D Tripathi, Kumar, A and Singh, S. “Oxidative Stress and Inflammation in Diabetic Complications,” International Journal of Endocrinology, vol. 2014, Article ID 679754, 2 pages, 2014. doi:10.1155/2014/679754. Editorial
  31. Chauhan M, Rana A, Alex JM, Negi A, Singh S, Kumar R. Design, Microwave-mediated Synthesis and Biological Evaluation of Novel 4-Aryl(alkyl)amino-3-nitroquinoline and 2,4-Diaryl(dialkyl)amino-3-nitroquinolines as Anticancer Agents. Bioorg Chem. 2014.
  32. Kumar D, Kaur G, Negi A, Kumar S, Singh S and Kumar R. Synthesis and Xanthine Oxidase Inhibitory Activity of 5,6-Dihydropyrazolo/pyrazolo[1,5-c]quinazoline Derivatives. Bioorg Chem. 2014. 30;57C:57-64.
  33. Alex JM, Singh S, Kumar R. 1-Acetyl-3,5-diaryl-4,5-dihydro(1H)pyrazoles Exhibit Anticancer Activity Through Intracellular ROS Scavenging and Mitochondrial Dependent Death Pathway.Arch. Pharm. Chem. Life Sci. 2014. 347, 1–11.
  34. Dhar A, Mallick S, Ghosh P, Maiti A, Ahmed I, Bhattacharyya S, Mandal T, Manna A, Roy K, Singh S, Nayak DK, Wilder PT, Markowitz J, Weber D, Ghosh MK, Chattopadhyay S, Guha R, Konar A, Bandyopadhyay S, Roy S. Simultaneous Inhibition of Key Growth Pathways in Melanoma Cells and Tumor Regression by a Designed Bidentate Constrained Helical Peptide. Biopolymers. 2014. 101(4):344-58.. PMID: 24839139.
  35. Qi J, Singh S, Cai Q, Li L, Liu H, Vu H, Huang WJ, McDonald T, Lin A, Chang CI, Bhatia R and Kuo YH. Selective Targeting Of Inv(16)+ AML Stem Progenitor Cells By Inhibiting HDAC8. Blood. 2013. 122(21): 224.
  36. Baviskar AT, Banerjee UC, Gupta M, Singh R, Kumar S, Gupta MK, Kumar S, Raut SK, Khullar M, Singh S and Kumar R. Synthesis of Imine-pyrazolopyrimidinones and their Mechanistic Interventions on Anticancer Activity. Bioorganic & Medicinal Chemistry. 2013. 21(18): 5782-93.
  37. Singh S, Raina V, Dubash T, Sreenath K, Pavithra Land Chattopadhyay S. Regulation of GAD 65 promoter by SMAR1 and p53 upon Streptozotocin treatment.BMC Molecular Biology.2012.13: 28.
  38. Singh S, Sreenath K, Pavithra L, Roy S, and Chattopadhyay S. SMAR1 regulates free radical stress through modulation of AKR1a4 enzyme activity.Int J Biochem Cell Biol. 2010. 42(7): 1105-14.
  39. Sreenath K, Pavithra L, Singh S, Sinha S, Dash PK, Siddappa NB, Ranga U, Mitra D, and Chattopadhyay S. Nuclear matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling.Virology.2010.400(1):76.
  40. Pavithra L, Sreenath K, Singh S, and Chattopadhyay S. Heat-shock protein 70 binds to a novel sequence in 5' UTR of tumor suppressor SMAR1 and regulates its mRNA stability upon Prostaglandin A2 treatment.FEBS Lett. 2010. 584(6):1187.
  41. Kaul-Ghanekar R,Singh S, Mamgain H, Jalota-Badhwar A, Paknikar KM, and Chattopadhyay S. Tumor suppressor protein SMAR1 modulates the roughness of cell surface: combined AFM and SEM study.BMC Cancer. 2009; 9: 350.
  42. Pavithra L, Singh S, Sreenath K, and Chattopadhyay S. Tumor suppressor SMAR1 downregulates Cytokeratin 8 expression by displacing p53 from its cognate site.Int J Biochem. Cell Biol. 2009. 41(4): 862-71.
Books & Book Chapters :
  1. Topoisomerase Inhibitors: Classification, Mechanisms of Action and Adverse Effects EditorsRaj Kumar and Sandeep Singh.Nova Publishers, ISBN 978-1-53611-841-4).
  2. Kaur. G, Joshi. G, Sharma. P, Kumar. R, Singh S.,(2017).Topoisomerases Genetics and Its Associated Diseases. Nova Publishers, ISBN 978-1-53611-841-4. (CHAPTER)
  3. Joshi. G, Thakur. A, Kaur. G, Kalra. S, Singh S, Kumar. R., (2017).Interaction between Topoisomerases and Histone Deacetylases: Role in Cancer Progression and Therapeutic Intervention. Nova Publishers, ISBN 978-1-53611-841-4. (CHAPTER)
Patents :
  1. Title: Novel fused heterocycles and method of use and manufacture thereof. Inventors: Kumar, R., Singh S, Chauhan, M. Indian Patent application no. 201611014161 Published on 2/2/18.
  2. Title: Indazolo [2,3-C] quinazoline based flourophores and their applications in bio imaging and tagging: Inventors: Singh S,Kumar R, Joshi G. Indian patent application no. 201811028230 submitted on July 27, 2018
Relevant Scientific Skills :
  • Cell culture and related assays:2D/3D/Embroid body culture; mouse embryo fibroblast, T Cells, monocytes and other primary cell culture; Fluorescence, confocal, live cell imaging (Nikon, Carl Zeiss, Olympus) and Atomic Force microscopy (WiTech) with hands on exposure to High Content Analysis system (Perkin-Elmer); Flow cytometry assays; Purification of Nuclear Matrix, mitochondria and other cellular organelles; Production of polyclonal antibodies in Rabbits and standardization.
  • Genomics and transcriptomics assays:Genomic analysis (cloning, elaboration of restriction maps, gene structures analysis, reconstruction of   genomic DNAs); kit free small RNA analysis using qPCR; RNase protection assays, RNA/DNA pull down assays, RNA-IP (RIP); studies of DNA-protein or RNA-protein interactions by Gel Shift assays, South-Western blotting, in-vitro and in-vivo Matrix binding assays and chromatin immunoprecipitation (ChIP) assays, Southern and Norther assays.
  • Proteomics assays: Western blotting; Protein-protein interaction studies using yeast two hybrid, pull down, co-precipitation, immunoprecipitation and fluorescence anisotropy; processing of samples and analysis for protein identification and post-translational modification using Mass Spectrometry and 2-D gel electrophoresis
  • Other Molecular Biology assays:Purification of histones and in vitro chromatin assembly studies; HDAC; kinase and acetylation; Topoisomerase assays; Assays related to various enzymes involved in metabolism, ROS production etc.
  • Cellular Respiration assays: Kit based OXPHOS, glycolysis assays; respiration measurement using Oroboros O2K.